Cytotoxicity Assay Analyzer

SkillDev tools

Nanotoxicology skill for in vitro cytotoxicity assessment and cell viability analysis

Instructions available. Your AI can read the instructions. Execution depends on the setup they require.

Account requirements not reviewed. Check the skill instructions before use; ahel provides instructions and does not run this skill.

Add ahel to your AI once: Claude, ChatGPT, Cursor, Claude Code or Codex. Then ask it to use this.

Then ask your AI: use the Cytotoxicity Assay Analyzer skill

What this skill tells your AI

The instructions your AI receives, as published by a5c-ai/babysitter in library/specializations/domains/science/nanotechnology/skills/cytotoxicity-assay-analyzer/SKILL.md and read by ahel’s review.

Purpose

The Cytotoxicity Assay Analyzer skill provides comprehensive in vitro toxicity assessment of nanomaterials, enabling systematic evaluation of cell viability, determination of safe exposure levels, and identification of toxicity mechanisms.

Capabilities

MTT/MTS/WST assay analysis
Live/dead staining quantification
IC50/EC50 calculation
Dose-response curve fitting
Cell morphology analysis
Apoptosis/necrosis detection

Usage Guidelines

Cytotoxicity Assessment

Assay Selection
- Choose appropriate viability assay
- Consider nanoparticle interference
- Include proper controls
Data Analysis
- Calculate percent viability
- Fit dose-response curves
- Determine IC50/EC50
Result Interpretation
- Compare to benchmark materials
- Assess mechanism of toxicity
- Report with appropriate context

Process Integration

Nanomaterial Safety Assessment Pipeline
Nanoparticle Drug Delivery System Development

Input Schema

{
  "nanomaterial": "string",
  "cell_line": "string",
  "assay_type": "mtt|mts|wst|live_dead|ldh",
  "concentrations": ["number"],
  "exposure_time": "number (hours)"
}

Output Schema

{
  "viability_data": [{
    "concentration": "number",
    "viability": "number (%)",
    "std_dev": "number"
  }],
  "dose_response": {
    "ic50": "number",
    "ic50_unit": "string",
    "hill_slope": "number",
    "r_squared": "number"
  },
  "toxicity_classification": "string",
  "mechanism_indicators": ["string"]
}

Signals

GitHub stars: 2k
Forks: 112
Last commit: Sep 2026

Advanced

Item type: skill
Key: cytotoxicity-assay-analyzer
Source: github.com/a5c-ai/babysitter

github.com/a5c-ai/babysitter